DNA

Part:BBa_K4342013:Design

Designed by: Keaton Brown   Group: iGEM22_Austin_UTexas   (2022-10-04)


recJ Upstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to ligate to the tdk/kan cassette and the recJ downstream homology via bsaI and bsmbI digestions.


Source

Acinetobacter baylyi ADP1


References